| 质粒名称: | pBABE hBRM |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 1961 |
| 存储实验室: | Robert Kingston |
|
载体骨架基本信息 |
|
| 载体名称: | pBABE-puro |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5200 bp |
| 载体类型: | 哺乳细胞表达，逆转录病毒 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | BRM |
| 物种来源: | 人源 |
| 基因又名: | BAF190b |
| 标签/融合蛋白: | flag (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | EcoRI (未破坏) |
| 5' 测序引物 | pBABE 5' |
|
文献引用方法 |
|
| 材料和方法部分: | pBABE hBRM was a gift from Robert Kingston (爱迪基因 plasmid # 1961) |
| 参考文献部分: | Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes. Sif S, Saurin AJ, Imbalzano AN, Kingston RE. Genes Dev 2001 Mar 1;15(5):603-18. 10.1101/gad.872801 PubMed 11238380 |
买家导航
pBABE hBRM载体质粒基本信息
pBABE hBRM质粒图谱载体图谱和pBABE hBRM载体序列质粒序列多克隆位点信息
pBABE hBRM质粒载体简介
pBABE hBRM质粒序列载体序列
LOCUS pBABE hBRM 10054 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBABE hBRM
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10054)
AUTHORS Sif S, Saurin AJ, Imbalzano AN, Kingston RE
TITLE Purification and characterization of mSin3A-containing Brg1 and hBrm
chromatin remodeling complexes.
JOURNAL Genes Dev 2001 Mar 1;15(5):603-18.
PUBMED 11238380
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pBABE hBRM|
FEATURES Location/Qualifiers
source 1..10054
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1..417
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 347..369
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 385..401
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 5384..5407
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
primer_bind complement(5443..5463)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 5448..5777
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 5628..5763
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5690..5709
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5787..6386
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(5787..5806)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6283..6303
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 6607..7076
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(7206..7223)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7377..7965)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7457..7476)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8125..8985)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8748..8767
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8986..9090)
/gene="bla"
/label=AmpR promoter
LTR 9121..9590
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 9655..9854
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
ORIGIN
1 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
61 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccggc
421 cggatcccag tgtggtggta cgtaggaatt ccggatgctc agatgaaagc cccgagatca
481 cagagacccg gcgagatcac agagacccgg cctgaaggaa cgtggaaaga ccaatgtacc
541 tgttttgacc ggttgcctgg agcaagaagt tccagttggg gagaattttc agaagataaa
601 gtcggagatt gtggaaagac ttgacttgca gcattactct actgactggc agagacagga
661 gaggtagatg tcaacgccca cagaccctgg tgcgatgccc cacccagggc cttcgccggg
721 gcctgggcct tcccctgggc caattcttgg gcctagtcca ggaccaggac catccccagg
781 ttccgtccac agcatgatgg ggccaagtcc tggacctcca agtgtctccc atcctatgcc
841 gacgatgggg tccacagact tcccacagga aggcatgcat caaatgcata agcccatcga
901 tggtatacat gacaagggga ttgtagaaga catccattgt ggatccatga agggcactgg
961 tatgcgacca cctcacccag gcatgggccc tccccagagt ccaatggatc aacacagcca
1021 aggttatatg tcaccacacc catctccatt aggagcccca gagcacgtct ccagccctat
1081 gtctggagga ggcccaactc cacctcagat gccaccaagc cagccggggg ccctcatccc
1141 aggtgatccg caggccatga gccagcccaa cagaggtccc tcacctttca gtcctgtcca
1201 gctgcatcag cttcgagctc agattttagc ttataaaatg ctggcccgag gccagcccct
1261 ccccgaaacg ctgcagcttg cagtccaggg gaaaaggacg ttgcctggct tgcagcaaca
1321 acagcagcag caacagcagc agcagcagca gcagcagcag cagcagcagc agcaacagca
1381 gccgcagcag cagccgccgc aaccacagac gcagcaacaa cagcagccgg cccttgttaa
1441 ctacaacaga ccatctggcc cggggccgga gctgagcggc ccgagcaccc cgcagaagct
1501 gccggtgccc gcgcccggcg gccggccctc gcccgcgccc cccgcagccg cgcagccgcc
1561 cgcggccgca gtgcccgggc cctcagtgcc gcagccggcc ccggggcagc cctcgcccgt
1621 cctccagctg cagcagaagc agagccgcat cagccccatc cagaaaccgc aaggcctgga
1681 ccccgtggaa attctgcaag agcgggaata cagacttcag gcccgcatag ctcataggat
1741 acaagaactg gaaaatctgc ctggctcttt gccaccagat ttaagaacca aagcaaccgt
1801 ggaactaaaa gcacttcggt tactcaattt ccagcgtcag ctgagacagg aggtggtggc
1861 ctgcatgcgc agggacacga ccctggagac ggctctcaac tccaaagcat acaaacggag
1921 caagcgccag actctgagag aagctcgcat gaccgagaag ctggagaagc agcagaagat
1981 tgagcaggag aggaaacgcc gtcagaaaca ccaggaatac ctgaacagta ttttgcaaca
2041 tgcaaaagat tttaaggaat atcatcggtc tgtggccgga aagatccaga agctctccaa
2101 agcagtggca acttggcatg ccaacactga aagagagcag aagaaggaga cagagcggat
2161 tgaaaaggag agaatgcggc gactgatggc tgaagatgag gagggttata gaaaactgat
2221 tgatcaaaag aaagacaggc gtttagctta ccttttgcag cagaccgatg agtatgtagc
2281 caatctgacc aatctggttt gggagcacaa gcaagcccag gcagccaaag agaagaagaa
2341 gaggaggagg aggaagaaga aggctgagga gaatgcagag ggtggggagt ctgccctggg
2401 accggatgga gagcccatag atgagagcag ccagatgagt gacctccctg tcaaagtgac
2461 tcacacagaa accggcaagg ttctgttcgg accagaagca cccaaagcaa gtcagctgga
2521 cgcctggctg gaaatgaatc ctggttatga agttgcccct agatctgaca gtgaagagag
2581 tgattctgat tatgaggaag aggatgagga agaagagtcc agtaggcagg aaaccgaaga
2641 gaaaatactc ctggatccaa atagcgaaga agtttctgag aaggatgcta agcagatcat
2701 tgagacagct aagcaagacg tggatgatga atacagcatg cagtacagtg ccaggggctc
2761 ccagtcctac tacaccgtgg ctcatgccat ctcggagagg gtggagaaac agtctgccct
2821 cctaattaat gggaccctaa agcattacca gctccagggc ctggaatgga tggtttccct
2881 gtataataac aacttgaacg gaatcttagc cgatgaaatg gggcttggaa agaccataca
2941 gaccattgca ctcatcactt atctgatgga gcacaaaaga ctcaatggcc cctatctcat
3001 cattgttccc ctttcgactc tatctaactg gacatatgaa tttgacaaat gggctccttc
3061 tgtggtgaag atttcttaca agggtactcc tgccatgcgt cgctcccttg tcccccagct
3121 acggagtggc aaattcaatg tcctcttgac tacttatgag tatattataa aagacaagca
3181 cattcttgca aagattcggt ggaaatacat gatagtggac gaaggccacc gaatgaagaa
3241 tcaccactgc aagctgactc aggtcttgaa cactcactat gtggccccca gaaggatcct
3301 cttgactggg accccgctgc agaataagct ccctgaactc tgggccctcc tcaacttcct
3361 cctcccaaca atttttaaga gctgcagcac atttgaacaa tggttcaatg ctccatttgc
3421 catgactggt gaaagggtgg acttaaatga agaagaaact atattgatca tcaggcgtct
3481 acataaggtg ttaagaccat ttttactaag gagactgaag aaagaagttg aatcccagct
3541 tcccgaaaaa gtggaatatg tgatcaagtg tgacatgtca gctctgcaga agattctgta
3601 tcgccatatg caagccaagg ggatccttct cacagatggt tctgagaaag ataagaaggg
3661 gaaaggaggt gctaagacac ttatgaacac tattatgcag ttgagaaaaa tctgcaacca
3721 cccatatatg tttcagcaca ttgaggaatc ctttgctgaa cacctaggct attcaaatgg
3781 ggtcatcaat ggggctgaac tgtatcgggc ctcagggaag tttgagctgc ttgatcgtat
3841 tctgccaaaa ttgagagcga ctaatcaccg agtgctgctt ttctgccaga tgacatctct
3901 catgaccatc atggaggatt attttgcttt tcggaacttc ctttacctac gccttgatgg
3961 caccaccaag tctgaagatc gtgctgcttt gctgaagaaa ttcaatgaac ctggatccca
4021 gtatttcatt ttcttgctga gcacaagagc tggtggcctg ggcttaaatc ttcaggcagc
4081 tgatacagtg gtcatctttg acagcgactg gaatcctcat caggatctgc aggcccaaga
4141 ccgagctcac cgcatcgggc agcagaacga ggtccgggta ctgaggctct gtaccgtgaa
4201 cagcgtggag gaaaagatcc tcgcggccgc aaaatacaag ctgaacgtgg atcagaaagt
4261 gatccaggcg ggcatgtttg accaaaagtc ttcaagccac gagcggaggg cattcctgca
4321 ggccatcttg gagcatgaag aggaaaatga ggaagaagat gaagtaccgg acgatgagac
4381 tctgaaccaa atgattgctc gacgagaaga agaatttgac ctttttatgc ggatggacat
4441 ggaccggcgg agggaagatg cccggaaccc gaaacggaag ccccgtttaa tggaggagga
4501 tgagctgccc tcctggatca ttaaggatga cgctgaagta gaaaggctca cctgtgaaga
4561 agaggaggag aaaatatttg ggagggggtc ccgccagcgc cgtgacgtgg actacagtga
4621 cgccctcacg gagaagcagt ggctaagggc catcgaagac ggcaatttgg aggaaatgga
4681 agaggaagta cggcttaaga agcgaaaaag acgaagaaat gtggataaag atcctgcaaa
4741 agaagatgtg gaaaaagcta agaagagaag aggccgccct cccgctgaga aactgtcacc
4801 aaatcccccc aaactgacaa agcagatgaa cgctatcatc gatactgtga taaactacaa
4861 agatagttca gggcgacagc tcagtgaagt cttcattcag ttaccttcaa ggaaagaatt
4921 accagaatac tatgaattaa ttaggaagcc agtggatttc aaaaaaataa aggaaaggat
4981 tcgtaatcat aagtaccgga gcctaggcga cctggagaag gatgtcatgc ttctctgtca
5041 caacgctcag acgttcaacc tggagggatc ccagatctat gaagactcca tcgtcttaca
5101 gtcagtgttt aagagtgccc ggcagaaaat tgccaaagag gaagagagtg aggatgaaag
5161 caatgaagag gaggaagagg aagatgaaga agagtcagag tccgaggcaa aatcagtcaa
5221 ggtgaaaatt aagctcaata aaaaagatga caaaggccgg gacaaaggga aaggcaagaa
5281 aaggccaaat cgaggaaaag ccaaacctgt agtgagcgat tttgacagcg atgaggagca
5341 ggatgaacgt gaacagtcag aaggaagtgg gacggatgat gaggactaca aggacgacga
5401 tgacaaatga taagaattcg ccagcacagt ggtcgaccct gtggaatgtg tgtcagttag
5461 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
5521 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
5581 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
5641 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
5701 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
5761 gcctaggctt ttgcaaaaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
5821 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
5881 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
5941 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
6001 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
6061 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
6121 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
6181 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
6241 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
6301 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
6361 gcatgacccg caagcccggt gcctgacgcc cgccccacga cccgcagcgc ccgaccgaaa
6421 ggagcgcacg accccatgca tcgataaaat aaaagatttt atttagtctc cagaaaaagg
6481 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
6541 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
6601 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
6661 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt
6721 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt
6781 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
6841 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
6901 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg
6961 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct
7021 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg
7081 cagcatgtat caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag
7141 ttgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
7201 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
7261 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
7321 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
7381 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
7441 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
7501 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
7561 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
7621 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
7681 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
7741 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
7801 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
7861 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
7921 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
7981 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
8041 agattatcaa aaaggatctt cacctagatc cttttgcggc cgcaaatcaa tctaaagtat
8101 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
8161 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
8221 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
8281 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
8341 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
8401 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
8461 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
8521 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
8581 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
8641 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
8701 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
8761 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
8821 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
8881 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
8941 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
9001 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
9061 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgcagc
9121 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
9181 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
9241 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
9301 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
9361 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
9421 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
9481 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
9541 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
9601 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggcaagctg
9661 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
9721 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
9781 gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg
9841 tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct
9901 ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg
9961 gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg
10021 tctgactgtg tttctgtatt tgtctgaaaa ttag
//
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pBABE hBRM质粒载体应用举例
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